N-cadherin in adult rat cardiomyocytes in culture. II. Spatio-temporal appearance of proteins involved in cell-cell contact and communication. Formation of two distinct N-cadherin/catenin complexes.
نویسندگان
چکیده
The spatio-temporal appearance and distribution of proteins forming the intercalated disc were investigated in adult rat cardiomyocytes (ARC). The 'redifferentiation model' of ARC involves extensive remodelling of the plasma membrane and of the myofibrillar apparatus. It represents a valuable system to elucidate the formation of cell-cell contact between cardiomyocytes and to assess the mechanisms by which different proteins involved in the cell-cell adhesion process are sorted in a precise manner to the sites of function. Appearance of N-cadherin, the catenins and connexin43 within newly formed adherens and gap junctions was studied. Here first evidence is provided for a formation of two distinct and separable N-cadherin/catenin complexes in cardiomyocytes. Both complexes are composed of N-cadherin and alpha-catenin which bind to either beta-catenin or plakoglobin in a mutually exclusive manner. The two N-cadherin/catenin complexes are assumed to be functionally involved in the formation of cell-cell contacts in ARC; however, the differential appearance and localization of the two types of complexes may also point to a specific role during ARC differentiation. The newly synthesized beta-catenin containing complex is more abundant during the first stages in culture after ARC isolation, while the newly synthesized plakoglobin containing complex progressively accumulates during the morphological changes of ARC. ARC formed a tissue-like pattern in culture whereby the new cell-cell contacts could be dissolved through Ca2+ depletion. Presence of cAMP and replenishment of Ca2+ content in the culture medium not only allowed reformation of cell-cell contacts but also affected the relative protein ratio between the two N-cadherin/catenin complexes, increasing the relative amount of newly synthesized beta-catenin over plakoglobin at a particular stage of ARC differentiation. The clustered N-cadherin/catenin complexes at the plasma membrane appear to be a prerequisite for the following gap junction formation; a temporal sequence of the appearance of adherens junction proteins and of gap junctions forming connexin-43 is suggested.
منابع مشابه
The effect of 4 weeks of endurance training and eugenol consumption on the expression of Gap43 and E-cadherin genes in the heart tissue of chlorpyrifos-poisoned mice
1.Introduction Among the various tissues of the body, the heart is one of the main and most important organs of the body to which human life depends, and any tissue and physiological damage to it disrupts human health (10) . On the other hand, today, prescribing sports activities is accepted as an effective prescription in the prevention and treatment of many diseases and improving heart f...
متن کاملBeta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells
Background: The canonical Wnt signal transduction (or the Wnt/β-catenin pathway) plays a crucial role in the development of animals and in carcinogenesis. Beta-catenin is the central component of this signaling pathway. The activation of Wnt/β-catenin signaling results in the cytoplasmic and nuclear accumulation of β-catenin. In the nucleus, β-catenin interacts with the TCF/LEF transcription fa...
متن کاملAssociation of p120, a tyrosine kinase substrate, with E- cadherin/catenin complexes
p120 was originally identified as a substrate of pp60src and several receptor tyrosine kinases, but its function is not known. Recent studies revealed that this protein shows homology to a group of proteins, beta-catenin/Armadillo and plakoglobin (gamma-catenin), which are associated with the cell adhesion molecules cadherins. In this study, we examined whether p120 is associated with E-cadheri...
متن کاملPlakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin
E- and N-cadherin are members of a family of calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, the transmembrane cadherins self-associate, while, intracellularly, they interact with the actin-based cytoskeleton. Several intracellular proteins, collectively termed catenins, have been noted to co-immunoprecipitate with E- and N-cadherin and are thought...
متن کاملA comparative evaluation on E-cadherin expression in oral squamous cell carcinoma with and without regional lymph node metastasis
A comparative evaluation on E-cadherin expression in oral squamous cell carcinoma with and without regional lymph node metastasis Dr. N. Mohtasham* - Dr. A. Omidi**- Dr. K. Kamyabhesari*** - Dr. M. Shakeri**** *- Assistant Professor of Oral Pathology Dept. - Faculty of Dentistry – Mashhad University of Medical Sciences. **- Professor of Pathology Dept. – Ghaem Hospital. ***- Fellowship of Derma...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of cell science
دوره 109 ( Pt 1) شماره
صفحات -
تاریخ انتشار 1996